Cerca Articoli

Loss of the Otx2-Binding Site in the Nanog Promoter Affects the Integrity of Embryonic Stem Cell Subtypes and Specification of Inner Cell Mass-Derived Epiblast

• Otx2 binds to the promoter/enhancer region of Oct4, Sox2, and Nanog in ESCs and EpiSCs • Otx2 binding to the Nanog promoter helps maintain the integrity of ESC compartments • Loss of this Otx2-binding site induces primed-like features in ESCs • Otx2 regulation of Nanog contributes to ICM differentiation of the epiblast Mouse embryonic stem cells (ESCs) and the inner cell mass (ICM)-derived epiblast exhibit naive pluripotency. ESC-derived epiblast stem cells (EpiSCs) and the postimplantation epiblast exhibit primed pluripotency. Although core pluripotency factors are well-characterized, additional regulators, including Otx2, recently have been shown to function during the transition from naive to primed pluripotency. Here we uncover a role for Otx2 in the control of the naive pluripotent state. We analyzed Otx2-binding activity in ESCs and EpiSCs and identified Nanog, Oct4, and Sox2 as direct targets. To unravel the Otx2 transcriptional network, we targeted the strongest Otx2-binding site in the Nanog promoter, finding that this site modulates the size of specific ESC-subtype compartments in cultured cells and promotes Nanog expression in vivo, predisposing ICM differentiation to epiblast. Otx2-mediated Nanog regulation thus contributes to the integrity of the ESC state and cell lineage specification in preimplantation development.

Recall of Ferrari 488 GTB for another fire risk?

Fuel lines and Ferraris: a fiery combination that has resulted in numerous Maranello mid-mounted engines ending up on the internet for the wrong publicity. Just recently the V8 in the latest Ferrari California T was recalled in certain affected markets and now it has been confirmed that the Ferrari 488 GTB has similar problems with the fuel lines.

Campaigning for the rights of the Batwa people of Burundi

Evariste Ndikumana is a member of the Batwa community from Burundi, in central Africa. With a population of 80,000, the Batwa are the third largest ethnic community in Burundi. Evariste recently represented the Batwa in a fellowship training program for French-speaking indigenous peoples organized by the UN Human Rights office in Geneva.

ALL ITALIANS SAY STOP TO AUTOSTRADE AND TELECOM MONOPOLY

ALL ITALIANS SAY STOP TO AUTOSTRADE AND TELECOM MONOPOLY. They rosds are of Demanio and Denaio Spa can give to anyone. The centre-left coalition under Romano Prodi has yet to form a government, and some ... But Autostrade, privatised in 1999, called the deal "a merger of equals". ...

Jef Raskin, Mac pioneer, dies at 61

Pacifica, CA February 27, 2005--Jef Raskin, a mathematician, orchestral soloist and composer, professor, bicycle racer, model airplane designer, and pioneer in the field of human-computer interactions, died peacefully at home in California on February 26th, 2005 surrounded by his family and loved ones. He had recently been diagnosed with pancreatic cancer.

NVIDIA Corporation: NVIDIA GPUs Selected for New Toshiba Notebooks

/PR Newswire/SANTA CLARA, Calif., NVIDIA Corporation (Nasdaq: NVDA), the worldwide leader in visual processing solutions, today announced that the Company's GeForce(TM) FX Go family has been selected for three notebooks from Toshiba Digital Products Division. The GeForce FX Go5200 graphics processing unit (GPU) has been selected for the new Toshiba Satellite M30-S350 and Portege M205. The GeForce FX Go5200 was also recently selected for Toshiba's Tecra M2 series. Perfectly balancing portability and performance, the Tecra M2 series weighs in at less than five pounds and is an excellent solution for business users who require small form factors and the compatibility, stability and reliability for which NVIDIA(R) is known.

Ghosts in the mind - Features - The Lab - Australian Broadcasting Corporation's Gateway to Science

Ghosts in the mind - Features - The Lab - Australian Broadcasting Corporation's Gateway to Science [ This is the print version of story ] Haunted Houses, silent spectres and bellowing banshees have startled and spooked innocent victims among the living for centuries. But does the world of the 'un-dead' really exist or do we create it all 'in-head'? Elaine Mulcahy enters the conflicting worlds of science and the supernatural to investigate.

THE FANCONI ANAEMIA/BRCA PATHWAY

Fanconi anaemia (FA) is a rare genetic cancer-susceptibility syndrome that is characterized by congenital abnormalities, bone-marrow failure and cellular sensitivity to DNA crosslinking agents. Seven FA-associated genes have recently been cloned, and their products were found to interact with well-known DNA-damage-response proteins, including BRCA1, ATM and NBS1. The FA proteins could therefore be involved in the cell-cycle checkpoint and DNA-repair pathways. Recent studies implicate the FA proteins in the process of repairing chromosome defects that occur during homologous recombination, and disruption of the FA genes results in chromosome instability ”€ a common feature of many human cancers.

Hair Multiplication Update - April 2002

DAVID BOWIE REVEALS 'HEATHEN'

First studio album in 3 years Hot on the heels of the news that his label, ISO Records, has inked a multi album deal with Columbia, David Bowie has announced track details for his forthcoming album 'HEATHEN' due for release June 10th.